Authors: Yan Shi Yongjia Tan Dongzhu Zeng Feng Qian Peiwu Yu
Publish Date: 2015/07/21
Volume: 37, Issue: 12, Pages: 15483-15488
Abstract
MicroRNAs miRNAs play critical roles in tumorigenesis and cancer metastasis Recently miR203 was reported as a tumor suppressor microRNA silenced in different malignancies including hepatocellular carcinoma prostate cancer oral cancer breast cancer and hematopoietic malignancy whereas its role in the carcinogenesis of gastric carcinoma GC has not been evaluated Here we analyzed the levels of miR203 and Slug in the GC specimen and studied their correlation We analyzed the binding of miR203 to the 3′UTR of Slug messenger RNA mRNA and its effects on Slug translation by bioinformatics analysis and by luciferasereporter assay respectively We modified miR203 levels in GC cells and studied their effects on the cell invasiveness in transwell cell migration assay We found that in GC miR203 levels were significantly decreased and Slug levels were significantly increased miR203 and Slug inversely correlated in patients’ specimen Bioinformatic analysis predicted that miR203 may target the 3′UTR of Slug mRNA to inhibit its translation which was confirmed by luciferasereporter assay Overexpression of miR203 inhibited Slug and cell invasiveness while depletion of miR203 increased Slug and cell invasiveness These data suggest that miR203 suppression in GC promotes Slugmediated cancer metastasis
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