Authors: Fangxuan Li Jinchao Huang Shixia Li Hui Li Jinpu Yu Xiubao Ren Juntian Liu
Publish Date: 2014/05/29
Volume: 35, Issue: 9, Pages: 8691-8698
Abstract
The abnormal distributions of memory T cells Tm and dendritic cells DC in stomach cancer are not well understood Indoleamine 23dioxygenase IDO produced by DC may be an important enzyme affecting function and proliferation of Tm In this study IDO expression was examined by immunohistochemical staining The subsets of Tm and DC were counted by flow cytometry The percentages of CD4 + Tm and CD4 + central Tm Tcm were lower in tumor tissues than in normal tissues P 005 while the CD4 + effector Tm Tem and CD8 + Tem percentages were higher in tumor tissues P 005 The ratio of myeloid DC DC1/plasmacytoid DC DC2 was significantly lower in tumor tissues P = 0009 The high expression of IDO was more frequently observed in tumor tissues P = 0001 The percentages of CD4 + Tm and CD8 + Tm were positively associated with DC1 percentage and ratio of DC1/DC2 P 005 The higher CD8 + Tcm percentage was associated with higher DC2 percentage P = 0025 The patients with high IDO expression had significantly lower CD4 + Tm P = 0012 and CD8 + Tm percentages P = 0033 but higher CD8 + Tem percentage P 001 Concerning on clinicopathologic features the higher DC2 percentage was associated with larger tumor size P = 0019 The CD4 + Tm and CD8 + Tem percentages were significantly associated with clinical stage and lymph node metastasis the high IDO expressions were significantly associated with deeper tumor invasion P = 0016 and lymph node metastasis P = 0038 Thus DC subsets Tm subsets and IDO expression were correlated with each other They were associated with the established clinicopathologic features such as tumor size depth of invasion lymph node metastasis and clinical stage
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