Authors: M Gokulnath N C Partridge N Selvamurugan
Publish Date: 2014/11/08
Volume: 36, Issue: 3, Pages: 1923-1931
Abstract
Activating transcription factor ATF3 is a stress response gene and is induced by transforming growth factor beta 1 TGFβ1 in breast cancer cells In this study we dissected the functional role of ATF3 gene in vitro by knocking down its expression stably in human bone metastatic breast cancer cells MDAMB231 Knockdown of ATF3 expression in these cells decreased cell number altered cell cycle phase transition and decreased mRNA expression of cell cycle genes Knockdown of ATF3 expression in MDAMB231 cells also decreased cell migration and the expression levels of invasive and metastatic genes such as MMP13 and Runx2 were found to be decreased in these cells Most importantly ATF3 was associated with Runx2 promoter in MDAMB231 cells and knockdown of ATF3 expression decreased its association with Runx2 promoter Hence our results suggested that ATF3 plays a role in proliferation and invasion of bone metastatic breast cancer cells in vitro and we identified for the first time that Runx2 is a target gene of ATF3 in MDAMB231 cell line
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