Authors: XiHua Chen WenGuang Wu Jian Ding
Publish Date: 2013/09/05
Volume: 35, Issue: 2, Pages: 967-971
Abstract
Recently it has been reported that tazaroteneinduced gene 1 TIG1 methylation was frequently detected in a variety of human cancers However the relationship between the TIG1 methylation and the characteristics of hepatocellular carcinoma HCC remains unknown The aim of present study was to observe the promoter methylation of TIG1 in HCC tissues and assess its prognostic significance for HCC Realtime quantitative polymerase chain reaction and methylationspecific polymerase chain reaction were used respectively to examine the mRNA expression and methylation status of TIG1 in 91 pairs of HCC and adjacent noncancerous tissues The mRNA expression level of TIG1 was significantly lower in HCC tissues than in adjacent noncancerous tissues The rate of TIG1 promoter methylation was significantly higher in HCC tissues than in adjacent noncancerous tissues P 0001 A strong correlation between downregulation and promoter methylation was found in these tumors P 0001 More importantly TIG1 methylation status was related to tumor size P = 0015 histological differentiation P = 0004 and tumor stage P 0001 Kaplan–Meier survival analysis showed that TIG1 promoter hypermethylation was associated with a worse outcome in patients with HCC Further Cox multivariate analysis indicated that TIG1 methylation status was an independent prognostic factor for the overall survival rate of HCC patients In conclusion our data suggested that epigenetic silencing of TIG1 gene expression by promoter hypermethylation may play an important role in HCC
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