Authors: Zhengqiang Wang Qianshan Ding Yanxia Li Qingqing Liu Wei Wu Lu Wu Honggang Yu
Publish Date: 2016/09/16
Volume: 37, Issue: 11, Pages: 14779-14787
Abstract
The aim of our study is to identify microRNAs miRNAs that have significance in the prognosis and pathogenesis of hepatocellular carcinoma HCC The miRNAs differentially expressed in HCC were examined by using a human miRNA microarray dataset and then the acquired candidates were screened by another microarray dataset As a result we got 25 miRNAs which were aberrantly expressed in cancer and meanwhile predicated distinct prognosis Among them miR1395p was downregulated in HCC and its low expression in cancer tissue meant poor prognosis Additionally we demonstrated that its low expression was also related to several clinicopathologic characteristics such as vein invasion BCLC stage pAKT expression and pIGFR1 expression In vitro it has been discovered that treatment of HCC cells with a miR1395p mimic lead to inhibition of cell growth and migration Moreover luciferase assay showed that KPNA4 was not the direct target of miR1395p Ectopic expression of miR1395p has not repressed the expression of KPNA4 but inhibited the nuclear import of NFκB and phosphorylation of Akt In conclusion for the first time we identify 25 deregulated miRNAs that are associated with prognosis and prove that miR1395p functions as a tumor suppressor in HCC and its low expression predicts poor prognosisThe authors thank all the colleagues in our laboratory for their suggestion and encouragement The first author is particularly grateful to Yang Hu a graduate of Kent State University for her language assistance The study was supported by the Natural Science Foundation of China No 81172350
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