Authors: Abdelfattah M Attallah Mohamed ElFar Camelia A Abdel Malak Mohamed M Omran Gamal E Shiha Khaled Farid Lamiaa A Barakat Mohamed S Albannan Ahmed A Attallah Mohamed A Abdelrazek Mohamed S Elbendary Refaat Sabry Gehan A Hamoda Mohamed M Elshemy Abdallah A Ragab Basma M Foda Sanaa O Abdallah
Publish Date: 2015/05/01
Volume: 36, Issue: 10, Pages: 7667-7674
Abstract
Currently the search for suitable hepatocellular carcinoma HCC biomarkers is very intensive Besides efficacy and cost/effectiveness of screening and surveillance of cirrhotics for the diagnosis of HCC is still debated So the present study is concerned with the evaluation of cytokeratin1 CK1 and nuclear matrix protein52 NMP52 for identifying HCC Twohundred and eighty individuals categorized into three groups liver fibrosis F1–F3 cirrhosis F4 and HCC constituted this study Western blot was used for identifying CK1 and NMP52 in serum samples As a result a single immunoreactive band was shown at 67 and 52 kDa corresponding to CK1 and NMP52 respectively Both CK1 and NMP52 bands were cut and electroeluted separately These markers were quantified in sera using ELISA Patients with HCC were associated with higher concentrations of CK1 and NMP52 than those without HCC with a significant difference P 00001 CK1 showed an area under receiveroperating characteristic curve AUC of 083 with 75 sensitivity and 82 specificity while NMP52 yielded 072 AUC with 62 sensitivity and 70 specificity for identifying HCC HCCDETECT comprising CK1 and NMP52 together with AFP was then constructed yielding 090 AUC for identifying HCC with 80 sensitivity and 92 specificity HCCDETECT was then tested for separating HCC from F1–F3 showing 094 AUC with 80 sensitivity and 93 specificity In conclusion CK1 in conjunction with NMP52 and AFP could have a potential role for improving the detection of HCC with a high degree of accuracy
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