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Title of Journal: Appl Phys A

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Abbravation: Applied Physics A

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Springer Berlin Heidelberg

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DOI

10.1007/s00339-008-4741-6

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ISSN

1432-0630

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Printing biological solutions through laserinduce

Authors: M Duocastella J M FernándezPradas J Domínguez P Serra J L Morenza
Publish Date: 2008/12/01
Volume: 93, Issue: 4, Pages: 941-945
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Abstract

Laserinduced forward transfer LIFT is a directwriting technique adequate for the highresolution printing of a wide range of materials including biological molecules In this article the preparation through LIFT of microarrays of droplets from a solution containing rabbit antibody immunoglobulin G IgG is presented The microarrays were prepared at different laser pulse energy conditions obtaining microdroplets with a circular and welldefined contour The transfer process has a double threshold a minimum energy density required to generate an impulsion on the liquid film and a minimum pulse energy which corresponds to the onset for material ejection In addition it was demonstrated that the transfer process can be correctly described through a simple model which relates the energy density threshold with the amount of released material Finally a fluorescence assay was carried out in which the preservation of the activity of the transferred biomolecules was demonstrated

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