Authors: Hiroshi Y Yoshikawa Ryota Murai Syou Maki Tomoya Kitatani Shigeru Sugiyama Gen Sazaki Hiroaki Adachi Tsuyoshi Inoue Hiroyoshi Matsumura Kazufumi Takano Satoshi Murakami Takatomo Sasaki Yusuke Mori
Publish Date: 2008/12/01
Volume: 93, Issue: 4, Pages: 911-915
Abstract
We have investigated femtosecond laserinduced nucleation of hen eggwhite lysozyme HEWL as a function of the laser pulse energy and pulse time width This is the first recorded study to confirm that the femtosecond laserinduced nucleation of HEWL can be induced at a specific threshold laser energy The threshold energy is comparable to that of cavitation bubble generation The results strongly suggest that morphological changes in the solution are key factors for protein nucleation
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