Authors: H J Alghanim J R Almirall
Publish Date: 2003/06/13
Volume: 376, Issue: 8, Pages: 1225-1233
Abstract
Microsatellite markers were developed for Cannabis sativa L marijuana to be used for DNA typing genotype identification and to measure the genetic relationships between the different plants Twelve different oligonucleotide probes were used to screen an enriched microsatellite library of Cannabis sativa in which 49 of the clones contained microsatellite sequences Characterization of microsatellite loci in Cannabis revealed that GA/CT was the most abundant class of the isolated microsatellites representing 50 overall followed by GTT/CAA AAG/TTC and GAT/CTA representing 16 15 and 10 respectively Eleven polymorphic STR markers were developed three derived from dinucleotide motifs and eight from trinucleotide motifs A total of 52 alleles were detected averaging 47 alleles/locus The expected heterozygosity of the eleven loci ranged between 0368 and 0710 and the common probability of identical genotypes was 18×10−7 The loci identified 27 unique profiles of the 41 Cannabis samples The 11 microsatellite markers developed in this study were found to be useful for DNA typing and for assessing genetic relatedness in CannabisThis work is part of the Masters thesis of the first author at Florida International University Miami Florida The authors would like to specially thank the Dubai Police Crime Laboratory in the United Arab Emirates for providing the financial support for this project and Drs Alan Meerow and Raymond Schnell at the USDAARSSHRS laboratory in Miami Florida for the use of their facilities and for helpful discussions and review of this manuscript The authors also thank Jason Clayton at the USDAARSSHRS laboratory for technical assistance and all the USDA staff at the genetic research lab in Miami Florida Finally the authors thank Dr Heather Coyle of CSFSL USA and Dr Gary Shutler of RCMP Canada for providing the DNA samples and the AFLP information
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