Authors: Wladimir A Benalcazar Stephen A Boppart
Publish Date: 2011/04/05
Volume: 400, Issue: 9, Pages: 2817-2825
Abstract
The most prevalent molecular constituents of skin are spatially mapped by the use of nonlinear interferometric vibrational imaging a coherent antiStokes Raman scattering CARSbased technique Ramanlike profiles over the range from 2800 to 3000 cm−1 are acquired by means of completely suppressing the nonresonant background allowing the generation of images based on the moleculespecific spectral profiles over the probed region with high spatial resolution A simple algorithm that maps spectral content to color allows the visualization of histology in a manner analogous to that obtained with more conventional staining procedures eg hematoxylineosin but faster and with the benefit of having access to localized spectra which could further enhance the potential for diagnosis of diseases especially during the early stages of developmentIdentification of adipose and collagenous domains in skin with Nonlinear Interferometric Vibrational Imaging a coherent antiStokes Raman scattering microscopy technique that excites vibrations over the bandwidth from 2800 to 3000 cm1 and with suppression of the nonresonant background by means of detecting the antiStokes signals interferometricallyWe are grateful to Eric Chaney for sectioning the tissue specimens and performing histological staining and to Dr Marina Marjanovic for important contributory discussions This work was supported in part by the National Institutes of Health National Cancer Institute R21/R33 CA115536 and a Beckman Institute for Advanced Science and Technology seed grant SAB W B was supported by a Beckman Institute graduate fellowship Additional information can be found at http//biophotonicsillinoisedu
Keywords: