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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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DOI

10.1016/0012-821x(70)90184-6

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1618-2650

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The goldnanoparticlebased surface plasmon resona

Authors: Xinyi Wang Yao Xu Yang Chen Limei Li Feng Liu Na Li
Publish Date: 2011/04/05
Volume: 400, Issue: 7, Pages: 2085-2091
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Abstract

We developed a new simple and sensitive assay for lysozyme based on gold nanoparticle plasmon resonance light scattering PRLS measurement and nakedeye detection using for the first time the lysozyme DNA aptamer as the recognition element Lysozyme DNA aptamer could stabilize gold nanoparticles AuNPs at high ionic strength Introducing lysozyme to the system easily triggered the aggregation of AuNPs producing a redtoblue color change of the solution redshifted plasmon absorption and enhanced plasmon resonance light scattering The linear range was found to be 02∼4 nM for 07 nM AuNPs 03∼6 nM for 14 nM AuNPs and 06∼8 nM for 21 nM AuNPs About 01 nM lysozyme can produce an observable enhancement of PRLS signal For visual detection 1 nM lysozyme can produce a very distinctive color change Satisfactory recoveries were obtained for simulated saliva and diluted urine samples indicating that the method has potential for analyses of clinical samples The simplicity and high sensitivity that are consistent with the resources and needs of many laboratories makes this method a good choice for routine analysis

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