Authors: Gong Cheng YanLin Liu JiLin Zhang DeHui Sun JiaZuan Ni
Publish Date: 2012/06/22
Volume: 404, Issue: 3, Pages: 763-770
Abstract
Novel Fe3O4La x Si y O5 affinity microspheres consisting of a superparamagnetic Fe3O4 core and an amorphous lanthanum silicate shell have been synthesized The core–shellstructured Fe3O4La x Si y O5 microspheres with a mean size of ca 480 nm had rough lanthanum silicate surfaces and displayed relatively strong magnetism 472 emu g−1 This novel affinity material can be used for selective capture rapid magnetic separation and part dephosphorylation which plays an important role in identifying phosphopeptides in MS of the phosphopeptides in a peptide mixture Its ability to selectively trap and magnetically isolate as well as label the phosphopeptides was evaluated using a standard phosphorylated protein βcasein and a real sample human serum Phosphopeptides and their corresponding label ions were detected for concentrations of βcasein as low as 1 × 10−9 M and in mixtures of βcasein and BSA with molar ratios as low as 150 In addition this affinity material with its labeling properties is superior to commercial TiO2 beads in terms of interference from nonphosphopeptide molecules These results reveal that the lanthanum silicate coated magnetic microspheres represent a promising affinity material for the rapid purification and recognition of phosphopeptidesA novel lanthanum silicate coated magnetic microspheres coreshell microsphere consisting of a superparamagnetic Fe3O4 core and an amorphous lanthanum silicate shell are synthesized This novel affinity material can be used for selective capture rapid magnetic separation and is wellsuited to the role of identifying phosphopeptides in a peptide mixture
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