Authors: Jens H Mohr Remco Swart Christian G Huber
Publish Date: 2011/04/17
Volume: 400, Issue: 8, Pages: 2391-2402
Abstract
The morphology of organic monolithic stationary phases based on polystyrenedivinylbenzene was modified by changing the ratio of monomers to microporogen in order to make them also suitable for small molecule separations The morphology of the columns was characterized by highresolution scanning electron micrography showing larger primary globules and larger macropores as well as no mesopores 20 nm in the monolithic skeleton The permeability of the modified monoliths was approximately three times higher than that of columns which have been optimized for large molecule separations enabling operation of a 30 cm long column at pressures below 250 bar In the isocratic separation of dansylated amino acids plate counts of 50000–107000 m−1 were achievable which are equivalent to efficiencies obtained with 31 μm porous particles The separation performance for small molecules in gradient elution was investigated using mixtures of dansylated amino acids βlactam antibiotics and thyroid hormones Finally the modified monolithic capillary columns also proved to be highly efficient in the separation of biopolymers such as peptides and proteins enabling peak width at half height of 3–8 s and peak capacities of 110–180 in 15–30 min gradient runsRecording of the SEM micrographs by Jörg Schmauch Institute of Technical Physics Saarland University Saarbrücken Germany is gratefully acknowledged as well as the supply of thyroid hormone samples by Ralf Braun Peptido GmbH Bexbach Germany This work was financially supported by the Priority Program Biosciences and Health of the University of Salzburg
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