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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1016/0022-3468(72)90082-6

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1618-2650

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Direct analysis of polyols using 3nitrophenylboro

Authors: Fan Fei Philip BritzMcKibbin
Publish Date: 2010/07/28
Volume: 398, Issue: 3, Pages: 1349-1356
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Abstract

The design of boronic acid sensors for photometric detection of carbohydrates has relied on exploiting differences in the thermodynamic stability of complex formation for molecular recognition Herein we introduce a direct method for analysis of sugar alcohols using 3nitrophenylboronic acid NPBA as an electrokinetic probe in capillary electrophoresis CE Dynamic complexation of neutral polyols by NPBA during electromigration allows for their simultaneous resolution and UV detection based on formation of an anionic ternary boronate ester complex in phosphate buffer Unlike conventional boronic acid sensors thermodynamic and electrokinetic processes in CE allow for improved selectivity for the resolution of sugar alcohol stereoisomers having different vicinal polyol chain lengths even in cases when binding affinity is similar due to differences in their complex mobility Three complementary approaches were investigated to compare the thermodynamics of polyol chelation with NPBA namely direct binding assays by CE UV absorbance spectroscopy and an indirect pK a depression method Overall CE offers a convenient platform for characterization of reversible arylboronic acid interactions in free solution while allowing for direct analysis of complex mixtures of neutral/UVtransparent polyols without complicated sample handling

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