Authors: Annika M Weber Huong Thi Huynh Nguyen Grady Hanrahan
Publish Date: 2013/12/14
Volume: 406, Issue: 5, Pages: 1447-1454
Abstract
This study demonstrates an untested link between model phenolic compounds and the formation/electrophoretic separation of stable urinary metabolites Sterically encumbered carbonyl groups were examined and mass determination was used to confirm the presence and stability of two oxidative metabolites of pentachlorophenol tetrachloro12benzoquinone and tetrachloro14dihydroquinone Subsequently baseline resolved separation of pentachlorophenol and the two oxidative metabolites was demonstrated under the following conditions 75 mM sodium tetraborate buffer pH = 85 with 5 methanol and 50 mM SDS +100 kV running voltage injection time = 50 s effective capillary length = 55 cm and run temperature = 20 °C Results not only provide key metabolic inferences for pentachlorophenol they also exhibit improvements in the ability to separate and detect changes in urinary metabolites in response to phenolicrelated exposureMetabolic pathway elucidation towards time and dosedependent electrophoretic screening of stable oxidative phenolic compounds Establishing direct pathways and developing sensitive/selective analytical tools to measure and characterize xenobiotics provides a defined link between potential hazards and suspected health effects
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