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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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DOI

10.1016/0022-0248(74)90213-9

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1618-2650

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Detection of bacteria by surfaceenhanced Raman sp

Authors: Atanu Sengupta Mirna Mujacic E James Davis
Publish Date: 2006/08/25
Volume: 386, Issue: 5, Pages: 1379-1386
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Abstract

The detection and identification of dilute bacterial samples by surfaceenhanced Raman spectroscopy has been explored by mixing aqueous suspensions of bacteria with a suspension of nanocolloidal silver particles An estimate of the detection limit of E coli was obtained by varying the concentration of bacteria By correcting the Raman spectra for the broad librational OH band of water reproducible spectra were obtained for E coli concentrations as low as approximately 103 cfu/mL To aid in the assignment of Raman bands spectra for E coli in D2O are also reportedThe authors would like to thank Professors Daniel T Schwartz and François Baneyx for their interest and advice The contributions of Daniel Allred in Professor Schwartz’s laboratory and undergraduate researcher Tian Huang are highly appreciated The authors would also like to thank the National Science Foundation for Grant Number CTS9982413 the University of Washington/PNNL Joint Institute for Nanotechnology and the University of Washington Center for Nanotechnology for financial support

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