Authors: Xiukun Xu Yunhui Yu Zheng Wang Tingting Zhu Yanping Wang Jian Zhu Zijun Chen Yun He Linling Ju Yunsen Li
Publish Date: 2013/04/18
Volume: 405, Issue: 15, Pages: 5267-5278
Abstract
In previous studies phospholipids have been proved to be involved in biochemical physiological and pathological processes As a special class of phospholipids peroxisomederived lipids PDLs have been proved to be potential ligands of invariant natural killer T iNKT cells in recent studies Here on the basis of phospholipidomics we focused on the relative quantity of PDLs extracted from mammalian thymus or bone marrow using electrospray ionization mass spectrometry MS In phospholipid analysis we identified 12 classes of phospholipids and accounted for their relative quantities by comparing their relative abundances in the MS1 map Our results show that PDLs are present in mammalian thymus as well as mouse spleen and liver Interestingly the relative quantity of PDLs extracted from human acute myeloid leukemia AML and acute lymphoblastic leukemia ALL bone marrows is higher than that extracted from bone marrow of healthy donors Our results may help to explain the close correlation between PDLs and iNKT cell function in thymus spleen liver and especially in leukemia patients We think that our phospholipidomics work may reveal a function of iNKT cells
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