Authors: Huiyan Zhang Tiejun Mi Oleg Yu Khan Yajie Sheng Sergei A Eremin Ross C Beier Suxia Zhang Jianzhong Shen Zhanhui Wang
Publish Date: 2015/08/16
Volume: 407, Issue: 25, Pages: 7843-7848
Abstract
Immunoglobulin Y IgY is derived from egg yolk and has been identified as a cheap and highyield immunoreagent The application of IgY in immunoassays for the detection of chemical contaminants in food samples has rarely been reported In this work we describe a rapid and sensitive fluorescence polarization immunoassay FPIA for valnemulin VAL using IgY which was produced using a previously prepared immunogen Three fluorophorelabeled VAL tracers were synthesized and the sensitivity of the best tracer VAL–DTAF in the optimized FPIA with antibody IgY100 demonstrated an IC50 value of 12 ng mL−1 in buffer After evaluation of several extraction procedures acidified acetonitrile was selected to extract VAL from swine tissue The recoveries of VAL in spiked swine tissue at three levels 50 100 and 200 μg kg−1 were higher than 79 with coefficients of variation CVs lower than 12 The limit of detection LOD of the FPIA in swine tissue was 26 μg kg−1 and was lower than the maximum residue limit MRL of VAL set by the European Union The study showed that IgY could be a good substitute for IgG when developing a highthroughput assay for chemical residues
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