Authors: Kiyoko Takamura Takatoshi Matsumoto
Publish Date: 2008/03/07
Volume: 391, Issue: 3, Pages: 951-961
Abstract
The Ti–TPyP reagent ie an acidic aqueous solution of the oxo5101520tetra4pyridylporphyrinato titaniumIV complex TiOtpyp was developed as a highly sensitive and selective spectrophotometric reagent for determination of traces of hydrogen peroxide Using this reagent determination of hydrogen peroxide was performed by flowinjection analysis with a detection limit of 05 pmol per test The method was actually applied to determination of several constituents of foods human blood and urine mediated by appropriate oxidase enzymes The reaction specificity of the TiOtpyp complex for hydrogen peroxide was clarified from the viewpoint of the reaction mechanisms and molecular orbitals based on ab initio calculations The results provided a wellgrounded argument for determination of hydrogen peroxide using the Ti–TPyP reagent experimentally This review deals with characterization of the high sensitivity and reaction specificity of the Ti–TPyP reagent for determination of hydrogen peroxide to prove its reliability in analytical applicationsThe authors are grateful to Emeritus Professor C Matsubara of Tokyo University of Pharmacy and Life Sciences for her collaboration throughout the experiments and to Professor P K Dasgupta of Texas University for his efforts to extend the application of the Ti–TPyP reagent to the measurement of the atmospheric hydrogen peroxide The authors thank Dr K Higuchi of Ogawa and Co Ltd formerly Tokyo Kasei Industries Co Ltd for his kind help in synthesizing the TiOtpyp complex at the beginning of these studies
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