Authors: Jie Zhan Guozhen Fang Zhen Yan Mingfei Pan Cuicui Liu Shuo Wang
Publish Date: 2013/06/18
Volume: 405, Issue: 19, Pages: 6353-6363
Abstract
In this work molecularly imprinted polymer MIP particles were synthesized using a semicovalent method based on a specific thermally reversible bond and these particles were used for the rapid detection of the azo dye acid orange II The imprinted polymers—which were prepared via the covalent reaction of 3triethoxysilylpropyl isocyanate with the template molecules—were attached to the surface of silicacoated magnetic nanoparticles and a simple thermal reaction was then performed to remove the templates leaving spaces with specific noncovalent bonds for target rerecognition The conditions for the synthesis of the MIP were optimized during the polymerization experiments to improve the adsorption capacity and selectivity The resulting polymers were characterized using Fourier transform infrared spectroscopy scanning electron microscopy and transmission electron microscopy to confirm their structure The MIPs were subjected to an online solidphase extraction and a magnetic molecularly imprinted solidphase extraction procedure For both methods all samples were prepared with spiking levels of 50 100 and 150 μg kg1 using highperformance liquid chromatography with UV/vis detection after the preconcentration of 50mL sample solutions the enhancement factors reached 710 and 629 The limits of detection signaltonoise ratio of 3 were 983 and 1741 ng L1 with relative standard deviations n = 9 of 672 and 825 respectively for a 40 μg L−1 standard template solution These two methods were developed to quantify trace acid orange II contents in food and environmental samples the recoveries ranged from 72 to 105 and from 70 to 94 respectivelyThis work was supported by the National Science Foundation for Distinguished Young Scholars of China project no 31225021 the Ministry of Science and Technology of China project no 2011BAD23B054 the Special Fund for Agroscientific Research in the Public Interest project no 201203069 Tianjin Municipal Science and Technology Commission project no 10SYSYJC28300 and the Program for Changjiang Scholars and Innovative Research Team in University project no IRT1166
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