Authors: Andreas Thomas Simon Beuck Jens Christian Eickhoff Sven Guddat Oliver Krug Matthias Kamber Wilhelm Schänzer Mario Thevis
Publish Date: 2010/03/12
Volume: 396, Issue: 8, Pages: 2899-2908
Abstract
Influencing the endurance in elite sports is one of the key points in modern sports science Recently a new class of prohibited substances reached in the focus of doping control laboratories and their misuse was classified as gene doping The adenosine monophosphate activated protein kinase activator 5amino4imidazolecarboxyamide ribonucleoside AICAR was found to significantly enhance the endurance even in sedentary mice after treatment Due to endogenous production of AICAR in healthy humans considerable amounts were present in the circulation and thus were excreted into urine Considering these facts the present study was initiated to fix reference values of renally cleared AICAR in elite athletes Therefore a quantitative analytical method by means of isotopedilution liquid chromatography analytical column C6phenyl coupled to tandem mass spectrometry after a sample preparation consisting of a gentle dilution of native urine was developed Doping control samples of 499 athletes were analysed and AICAR concentrations in urine were determined The mean AICAR value for all samples was 2186 ng/mL with a standard deviation of 1655 ng/mL Concentrations were found to differ depending on gender type of sport and type of sample collection in competition/out of competition The method was fully validated for quantitative purposes considering the parameters linearity inter 12 7 and 10 and intraday precision 14 9 and 12 at low mid and high concentration robustness accuracy approx 100 limit of quantification 100 ng/mL stability and ion suppression effects employing an inhouse synthesised 13C5labelled AICAR as internal standard
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