Authors: Jing Zhao Gang Li Baomin Wang Wei Liu Tiegui Nan Zhixi Zhai Zhaohu Li Qing X Li
Publish Date: 2006/09/28
Volume: 386, Issue: 6, Pages: 1735-
Abstract
Glycyrrhizic acid GL is a major active compound of licorice The specific monoclonal antibody MAb designated as 8F8A8H42H7 against GL was produced with the immunogen GL–BSA conjugate The dissociation constant K d value of the MAb was approximately 996×10−10 M The cross reactivity of the MAb with glycyrrhetic acid was approximately 26 The conventional indirect competitive enzymelinked immunosorbent assay icELISA and simplified icELISA adapted with a modified procedure were established using the MAb The IC50 value and the detect range by the conventional icELISA were 11 ng mL−1 and 02–51 ng mL−1 respectively The IC50 value and the detect range by the simplified icELISA were 53 ng mL−1 and 12–238 ng mL−1 respectively The two icELISA formats were used to analyze GL contents in the roots of wild licorice and different parts of cultivated licorice Glycyrrhiza uralensis Fisch The results obtained with the two icELISAs agreed well with those of the HPLC analysis The correlation coefficient was more than 098 between HPLC and the two icELISAs The two icELISAs were shown to be appropriate simple and effective for the quality control of raw licorice root materials
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