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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1016/1353-4858(94)90069-8

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1618-2650

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The good the bad and the tiny a review of micro

Authors: Daniel A Ateya Jeffrey S Erickson Peter B Howell Lisa R Hilliard Joel P Golden Frances S Ligler
Publish Date: 2008/01/29
Volume: 391, Issue: 5, Pages: 1485-1498
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Abstract

Recent developments in microflow cytometry have concentrated on advancing technology in four main areas 1 focusing the particles to be analyzed in the microfluidic channel 2 miniaturization of the fluidhandling components 3 miniaturization of the optics and 4 integration and applications development Strategies for focusing particles in a narrow path as they pass through the detection region include the use of focusing fluids nozzles and dielectrophoresis Strategies for optics range from the use of microscope objectives to polymer waveguides or optical fibers embedded onchip While most investigators use offchip fluidic control there are a few examples of integrated valves and pumps To date demonstrations of applications are primarily used to establish that the microflow systems provide data of the same quality as laboratory systems but new capabilities—such as automated sample staining—are beginning to emerge Each of these four areas is discussed in detail in terms of the progress of development the continuing limitations and potential future directions for microflow cytometersThe preparation of this manuscript was supported by NRL/ONR 62 Work Unit 6006 DAA and LRH are National Research Council Postdoctoral Fellows The views are those of the authors and do not reflect policy or opinion of the US Navy or Department of Defense

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