Authors: Hu Zhang Xinquan Wang Shulin Zhuang Mingrong Qian Kezhi Jiang Xiangyun Wang Hao Xu Peipei Qi Qiang Wang
Publish Date: 2012/09/06
Volume: 404, Issue: 6-7, Pages: 1983-1991
Abstract
A method for simultaneous enantioselective determination of fenarimol and nuarimol in apple grape cucumber tomato and soil was developed using liquid chromatography–tandem mass spectrometry The enantioseparation results of the two fungicides through three different cellulosebased chiral columns are discussed The influence of column temperature on the resolution of the enantiomers of the two fungicides was examined Complete enantioseparation of the two fungicides’ enantiomers was obtained on a cellulose tris4methylbenzoate column Lux Cellulose3 at 25 °C using methanol and 01 formic acid solution 8020 v/v as mobile phase The linearity matrix effect recovery and precision were evaluated Good linearity was obtained over the concentration range of 1–500 μg L−1 for each enantiomer in the standard solution and sample matrix calibration solution There was no significant matrix effect in apple grape cucumber or tomato samples but signal suppression was typically observed with the soil extracts The mean recoveries repeatability and reproducibility were 765–103 21–90 and 42–118 respectively The limit of quantification for enantiomers of the two fungicides in fruits vegetables and soil was 5 μg kg−1 Moreover the absolute configuration of the enantiomers of fenarimol and nuarimol was determined from a combination of experimentally determined and predicted electronic circular dichroism spectraThe authors gratefully acknowledge financial support from the National Natural Science Foundation of China no 21107094 the National High Technology RD Program of China The 863 Program no 2011AA100806 and the Fundamental Research Funds for the Central Universities
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