Authors: Hana Sklenářová Petr Chocholouš Petra Koblová Lukáš Zahálka Dalibor Šatínský Ludmila Matysová Petr Solich
Publish Date: 2012/12/04
Volume: 405, Issue: 7, Pages: 2255-2263
Abstract
This work describes a comparison of three types of commercial highperformance liquid chromatography silica monolithic columns with different inner diameters and generations of monolithic sorbent a “classic” monolithic column the first generation Onyx™ monolithic C18 100 mm × 46 mm Phenomenex a “narrow” monolithic column for fast separation at lower flow rates Chromolith® Performance RP18e 100 mm × 3 mm Merck and a recently introduced “highresolution” monolithic column the next generation Chromolith® HighResolution RP18e 100 mm × 46 mm Merck Separation efficiency number of theoretical plates height equivalent to a theoretical plate and van Deemter curves working pressure the symmetry factor and resolution were critical aspects of the comparison in the case of the separation of ascorbic acid paracetamol and caffeine The separations were performed under isocratic conditions with a mobile phase consisting of 1090 v/v acetonitrile–phosphoric acid pH 280 Detailed comparison of the newestgeneration monolithic column Chromolith® HighResolution with the previously introduced monolithic sorbents was performed and proved the advantages of the Chromolith® HighResolution columnThe authors express their thanks to Egidijus Machtejevas Analytical Chromatography Product Management Merck Millipore Darmstadt Germany for providing HRMCs This study was supported by the Charles University in Prague projects SVV2012/265 002 and 17/2012/UNCE and the Grant Agency of Charles University no 34609/2009 and was cofinanced by the European Social Fund and the state budget of the Czech Republic TEAB project no CZ107/2300/200235
Keywords: