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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1016/0198-0254(86)90970-2

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1618-2650

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Mass spectrometry based analysis of nucleotides n

Authors: Stefan Neubauer Ariana Rugova Dinh Binh Chu Hedda Drexler Anja Ganner Michael Sauer Diethard Mattanovich Stephan Hann Gunda Koellensperger
Publish Date: 2012/06/30
Volume: 404, Issue: 3, Pages: 799-808
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Abstract

In this work accurate MSbased methods for quantitative profiling of nucleotides nucleosides and nucleobases in yeast extracts used as additives in animal feedstuff are presented Reversedphase chromatography utilizing a stationary phase compatible with 100  aqueous mobile phases resulted in superior analytical figures of merit than HILIC or ionpair reversedphase separation The novel separation method was combined with both molecular and elemental mass spectrometry By use of RP–LC–MS–MS excellent limits of detection 1 μmol L−1 could be obtained for all the compounds investigated The elemental speciation analysis approach enabled determination of nucleotides by phosphorus detection Sensitivity of LC–ICP–MS was 1–2 orders of magnitude lower than that of LC–MS–MS Quantitative analysis of yeast products using complementary MS detection furnished values in good agreement

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