Authors: Ana Čevdek Mladen Franko
Publish Date: 2010/06/27
Volume: 398, Issue: 1, Pages: 555-562
Abstract
This work presents a comparison of convective interaction media CIM and controlled pore glass CPG as solid supports for immunoglobulin antibodies used in bioanalytical detection of allergens in foodstuffs A flowinjection manifold with highly sensitive thermal lens spectrometric detection was used for this purpose Using betalactoglobulin a milk allergen as a model analyte CIM disc supports had a higher linear range 02–35 μg L−1 better reproducibility intraday RSD = 1 interday RSD = 10 lower consumption of reagents and better immunocolumn stability 1 month over 240 injections of substrate while providing comparable LODs 01 μg L−1 Application of CIM discs as solid supports in immunocolumns for allergen detection enables fast and sensitive screening of allergens in foodstuffs with sample throughput of up to eight samples per hourThis work was financially supported by the Slovenian Research Agency through research program P10034 and a young researcher’s grant to Ana Čevdek Financial support for equipment was obtained from the EU and the Municipality of Nova Gorica through structural funds contract C153608S330265 The authors are also grateful to BIA Separations for their kind donation of diol CIM micro discs and to Dr Aleš Podgornik for inspiring discussions
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