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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer Berlin Heidelberg

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DOI

10.1016/0098-3004(77)90004-8

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1618-2650

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Analysis of free and bound phenolics in wine and g

Authors: David Allen Anh Duyen Bui Nicole Cain Gavin Rose Mark Downey
Publish Date: 2013/10/15
Volume: 405, Issue: 30, Pages: 9869-9877
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Abstract

The results of validation of a method for the analysis of free and bound phenolics in wine and grapes are presented Wine and grape extracts are fractionated by automated solidphase extraction on Bond Elut PPL cartridges to give free and bound phenolic fractions Bound fractions are subjected to acid hydrolysis and the phenolics released are recovered by solidphase extraction on Bond Elut PPL cartridges The fractions are further purified by automated solidphase extraction on Bond Elut silica cartridges After derivatisation to form trimethylsilyl ethers the phenolics are determined by gas chromatography–mass spectrometry with selected ion monitoring The method is suitable for robust highthroughput monitoring of the concentrations of phenolics that can affect the palatability of wineThe authors gratefully acknowledge the provision of Sauvignon Blanc grape and wine samples by David Kelly Department of Environment and Agriculture Curtin University Margaret River Western Australia and the kind gift of analytical standards by Dr Yoji Hayasaka The Australian Wine Research Institute Glen Osmond South Australia

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