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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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DOI

10.1002/bip.1978.360170604

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1618-2650

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Cross validation of multiple methods for measuring

Authors: Dana B Barr Gabriele Leng Edith BergerPreiß HansWolfgang Hoppe Gayanga Weerasekera Wolfgang Gries Susanne Gerling Jose Perez Kimberly Smith Larry L Needham Jürgen Angerer
Publish Date: 2007/09/09
Volume: 389, Issue: 3, Pages: 811-
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Abstract

The objective of our study was to compare three vastly different analytical methods for measuring urinary metabolites of pyrethroid and pyrethrum insecticides to determine whether they could produce comparable data and to determine if similar analytical characteristics of the methods could be obtained by a secondary laboratory This study was conducted as a part of a series of validation studies undertaken by the German Research Foundation’s Committee on the Standardization of Analytical Methods for Occupational and Environmental Medicine We compared methods using different sample preparation methods liquid–liquid extraction and solidphase extraction with and without chemical derivatization and different analytical detection methods gas chromatography–mass spectrometry single quadrupole gas chromatography–high resolution mass spectrometry magnetic sector in both electron impact ionization and negative chemical ionization modes and highperformance liquid chromatography–tandem mass spectrometry triple quadrupole with electrospray ionization Our cross validation proved that similar analytical characteristics could be obtained with any combination of sample preparation/analytical detection method and that all methods produced comparable analytical results on unknown urine samples

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