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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1016/j.ajem.2012.02.017

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1618-2650

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A dynamic continuousflow dialysis system with on

Authors: Jeerawan Promchan Juwadee Shiowatana
Publish Date: 2005/06/10
Volume: 382, Issue: 6, Pages: 1360-1367
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Abstract

A dynamic continuousflow dialysis CFD method with online electrothermal atomic absorption spectrometric ETAAS and pH measurements for study of simulated gastrointestinal digestion has been developed for prediction of iron bioavailability The method used to estimate mineral bioavailability was based on gastric digestion in a batch system then dynamic continuousflow intestinal digestion The intestinal digestion was performed in a dialysis bag placed inside a chamber containing a flowing stream of dialyzing solution Mineral concentration and dialysate pH were monitored by ETAAS and use of a pH meter respectively The amount of dialyzed minerals in the intestinal digestion stage was used to evaluate the dialyzability The dialysis profile and pH change can be used to understand or examine differences between the dialyzability of different food samples To test the proposed system it was used to estimate the iron dialyzability of different kinds of milk Iron dialyzability for powdered cow milk cereal milk and two brands of soymilk was found to be 17 204 249 and 377 respectively The developed CFD–ETAAS–pH system is a simple rapid and inexpensive tool for bioavailability studies especially for minerals at ultratrace levels

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