Authors: Komal Kedia Caitlin A Nichols Craig D Thulin Steven W Graves
Publish Date: 2015/09/08
Volume: 407, Issue: 28, Pages: 8543-8556
Abstract
Tissue proteomics has relied heavily on twodimensional gel electrophoresis for protein separation and quantification then single protein isolation trypsin digestion and mass spectrometric protein identification Such methods are predominantly used for study of highabundance fulllength proteins Tissue peptidomics has recently been developed but is still used to study the most highly abundant species often resulting in observation and identification of dozens of peptides only Tissue lipidomics is likewise new and reported studies are limited We have developed an “omics” approach that enables over 7000 lowmolecularweight lowabundance species to be surveyed and have applied this to human placental tissue Because the placenta is believed to be involved in complications of pregnancy its proteomic evaluation is of substantial interest In previous research on the placental proteome abundant highmolecularweight proteins have been studied Application of largescale global proteomics or peptidomics to the placenta have been limited and would be challenging owing to the anatomic complexity and broad concentration range of proteins in this tissue In our approach involving protein depletion capillary liquid chromatography and tandem mass spectrometry we attempted to identify molecular differences between two regions of the same placenta with only slightly different cellular composition Our analysis revealed 16 species with statistically significant differences between the two regions Tandem mass spectrometry enabled successful sequencing or otherwise enabled chemical characterization of twelve of these The successful discovery and identification of regional differences between the expression of lowabundance lowmolecular weight biomolecules reveals the potential of our approachThis work was supported by the Department of Chemistry and Biochemistry Brigham Young University The authors would like to extend their gratitude to several individuals who participated in parts of this study Dr Moana HopoateSitake Bruce Jackson Jody Jones Dr M Sean Esplin and the Mass Spectrometry Facility at BYU We gratefully acknowledge the support provided by Intermountain Health Care IHC hospitals in making placental tissue samples available
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