Authors: LiWei Qian XiaoLing Hu Ping Guan Bo Gao Dan Wang ChaoLi Wang Ji Li ChunBao Du WenQi Song
Publish Date: 2014/09/27
Volume: 406, Issue: 28, Pages: 7221-7231
Abstract
Thermal preparation of lysozymeimprinted microspheres was firstly investigated by using biocompatible ionic liquid IL as a thermal stabilizer The imprinted microspheres made with IL could obtain the good recognition ability to template protein whereas the imprinted polymer synthesized in the absence of it had a similar adsorption capacity to the nonimprinted one Furthermore the preparation conditions of imprinted polymers MIPs including the content of IL temperature of polymerization and types of functional monomers and crosslinkers were systematically analyzed via circular dichroism spectrum and activity assay The results illustrated that using hydroxyethyl acrylate as the functional monomer ethylene glycol dimethacrylate as the crosslinker 5 IL as the stabilizer and 75 °C as the reaction temperature could retain the structure of template protein as much as possible The obtained MIPs showed excellent recognition ability to the template protein with the separation factor and selectivity factor value of 430 and 221 respectively Consequently it is an effective way to accurately imprint and separate template protein by cooperatively using circular dichroism spectroscopy and activity assay during the preparation of protein MIPs The method of utilizing IL to stabilizing protein at high temperature would offer a good opportunity for various technologies to improve the development of macromolecules imprinting
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