Authors: A Radi M A EL Ries S Kandil
Publish Date: 2005/01/19
Volume: 381, Issue: 2, Pages: 451-455
Abstract
Spectral and electrochemical studies have been carried out on the interaction of pefloxacin with calf thymus doublestranded dsDNA The voltammetric behavior of pefloxacin was investigated at glassy carbon carbon paste and dsDNAmodified carbon paste electrodes using cyclic voltammetry Pefloxacin was oxidized yielding one irreversible oxidation peak The modification of the carbon paste surface with dsDNA allowed an accumulation process to take place for pefloxacin such that higher sensitivity was achieved compared with the bare surface The response was characterized with respect to ionic strength accumulation time pefloxacin concentration and other variables The stripping differential pulse voltammetric response showed a linear calibration curve in the range 10×10−7–10×10−5 mol l−1 with a detection limit of 50×10−8 mol l−1 at the dsDNA modified electrode The method was applied to the direct determination of pefloxacin in diluted urine samples
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