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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1002/elan.201600569

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1618-2650

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Quantification of Paraquat MPTP and MPPSuperscr

Authors: Bozena Winnik Dana B Barr Mona Thiruchelvam M Angela Montesano Eric K Richfield Brian Buckley
Publish Date: 2009/07/19
Volume: 395, Issue: 1, Pages: 195-201
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Abstract

Animal models consistent with the hypothesis of direct interaction of paraquat PQ and 1methyl4phenyl1236tetrahydropyridine MPTP with specific areas of the central nervous system have been developed to study Parkinson’s disease PD in mice These models have necessitated the creation of an analytical method for unambiguous identification and quantitation of PQ and structurally similar MPTP and 1methyl4phenylpyridinium ion MPP+ in brain tissue A method for determination of these compounds was developed using microwaveassisted solvent extraction MASE and liquid chromatography–mass spectrometry Extraction solvent and microwave conditions such as power and time were optimized to produce recoveries of 90 for PQ 78 for MPTP and 97 for its metabolite MPP+ The chromatographic separation was performed on a C8 column and detection was carried out using an ion trap as an analyzer with electrospray ionization Mass spectrometer parameters such as heated capillary temperature spray voltage capillary voltage and others were also optimized for each analyte Analysis was done in selective ionmonitoring SIM mode using m/z 186 for PQ m/z 174 for MPTP and m/z 170 for MPP+ The method detection limit for paraquat in matrix was 100 pg 40 pg for MPTP and 20 pg MPP+

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