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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1007/bf01957172

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1618-2650

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Chromatographic evaluation of polymers imprinted w

Authors: Christina Schirmer Hans Meisel
Publish Date: 2009/07/03
Volume: 394, Issue: 8, Pages: 2249-2255
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Abstract

To obtain a highly selective material for the antibiotic chloramphenicol which has several harmful side effects in humans different molecularly imprinted polymers MIPs were prepared In order to avoid a major traditional drawback associated with MIPs of residual template bleeding molecules that are structurally related to chloramphenicol were used as templates for polymer synthesis Chromatographic evaluation indicated that the employed template imparted a significant influence on the recognition properties of the corresponding polymer A strong retention of chloramphenicol under nonpolar elution conditions k = 6803 IF = 1772 and under aqueous elution conditions k = 9244 IF = 135 was achieved After chromatographic evaluation the MIP was utilized as the recognition sorbent in a solidphase extraction to determine chloramphenicol using either an organic or aqueous washing solvent Recoveries of nearly 100 from the chloramphenicol standard solution and nearly 90 from honey samples spiked with chloramphenicol were attained Furthermore the applicability of the MIP for sample cleanup was demonstrated

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