Authors: Krisztian Herman László Szabó Loredana F Leopold Vasile Chiş Nicolae Leopold
Publish Date: 2011/02/26
Volume: 400, Issue: 3, Pages: 815-820
Abstract
A new simple and effective approach for multianalyte sequential surfaceenhanced Raman scattering SERS detection in a flow cell is reported The silver substrate was prepared in situ by laserinduced photochemical synthesis By focusing the laser on the 320 μm inner diameter glass capillary at 05 ml/min continuous flow of 1 mM silver nitrate and 10 mM sodium citrate mixture a SERS active silver spot on the inner wall of the glass capillary was prepared in a few seconds The test analytes dacarbazine 42pyridylazoresorcinol PAR complex with CuII and amoxicillin were sequentially injected into the flow cell Each analyte was adsorbed to the silver surface enabling the recording of high intensity SERS spectra even at 2 s integration times followed by desorption from the silver surface and being washed away from the capillary Before and after each analyte passed the detection window citrate background spectra were recorded and thus no “memory effects” perturbed the SERS detection A good reproducibility of the SERS spectra obtained under flow conditions was observed The laserinduced photochemically synthesized silver substrate enables high Raman enhancement is characterized by fast preparation with a high success rate and represents a valuable alternative for silver colloids as SERS substrate in flow approaches
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