Authors: O Gökay D Kühner M Los F Götz U Bertsche K Albert
Publish Date: 2010/09/09
Volume: 398, Issue: 5, Pages: 2039-2047
Abstract
Using Radix imperatoriae the root of masterwort as an example we describe an efficient approach for the isolation identification and evaluation of bioactive plant components on an analytical scale The extraction of Radix imperatoriae with ethyl acetate was enhanced by the application of ultrasound oscillations This rhizome extract was applied to three pathogenic bacteria Bacillus cereus Escherichia coli and Staphylococcus aureus to determine its antimicrobial activity Disk diffusion was utilized to determine susceptibility The extract components were separated using a series of chromatography approaches semipreparative RPHPLC or RPHPLC on an analytical scale followed by testing All fractions were analyzed by LCUVESIMS and 600 MHz microcoil 1H NMR spectroscopy Among other findings in the fraction with the highest antibacterial activity we were able to identify oxypeucedanin and oxypeucedanin hydrate Subsequent analysis revealed that only oxypeucedanin hydrate had antibacterial activity whereas oxypeucedanin itself was inactive at the concentrations applied Furthermore oxypeucedanin hydrate appears to be largely or exclusively a byproduct of sample preparation since it is either not synthesized by the plant as a second metabolite or is produced by it in only very small quantities
Keywords: