Authors: Oriane Tascone Marina Shirshikova Céline Roy Uwe J Meierhenrich
Publish Date: 2014/10/26
Volume: 406, Issue: 30, Pages: 8041-8048
Abstract
Damascena and centifolia roses are cultivated worldwide for their petal extracts that contain key odorant ingredients of perfumes The analytical identification and quantification of pesticides in rose petals have never been described in the literature Here we report on a newly developed method using dispersive solidphase extraction dSPE cleanup followed by gas chromatographytandem mass spectrometry for the quantitative determination of multiresidue pesticides in rose petals Analytes were extracted from the matrix using acetonitrile and a mixture of salts containing magnesium sulfate sodium citrate sodium chloride and sodium sesquihydrate Samples were cleaned up twice by dSPE applying primary and secondary amines PSAs magnesium sulfate C18 and graphitized carbon black GCB Two fortification levels of 005 and 05 mg kg−1 were assessed for method validation purposes The obtained pesticide recoveries were in the range of 70–120 with a relative standard deviation RSD of less than 20 The newly developed method was allowed for the quantification of 57 pesticides residues It was applied to pesticide residue detection in rose petals from an organic field without treatment compared to those from a field with classic phytosanitary treatment using fungicide and/or insecticide We did not detect pesticide residues in rose petals from the organic field The classically treated samples of roses contained pesticides such as chlorpyriphos and methidathion which are in accordance with the previous application of these pesticides on the roses Insecticides were quantified at 005 mg kg−1 rose petal maximumWe thank the ‘Association Nationale de la Recherche et de la Technologie’ ANRT concerning the CIFRE funding for the PhD thesis of OT We acknowledge the financial and scientific support of Waters and International Flavors Fragrances LMR Naturals division
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