Authors: Barbara Roda Sonia Casolari Pierluigi Reschiglian Mara Mirasoli Patrizia Simoni Aldo Roda
Publish Date: 2009/03/17
Volume: 394, Issue: 4, Pages: 953-961
Abstract
In this work the biospecific recognition antigen–antibody reaction was implemented in gravitational fieldflow fractionation GrFFF a flowassisted separation technique for micronsized particles in order to realize a hybrid immunomodulated GrFFF system in which two different principles are combined to achieve highly versatile fractionation Micronsized polystyrene beads coated with horseradish peroxidase HRP were used as a model sample and antiHRP antibodies were immobilized on the accumulation wall of the GrFFF channel Ultrasensitive chemiluminescence imaging was employed to visualize the beads during elution and to optimize experimental conditions The same principle was then applied to real biological samples composed by Yersinia enterocolitica and Escherichia coli cells Results show the possibility to modify the elution of selected sample components and even to retain them into the channel The hybrid immunomodulated GrFFF system is a step towards the development of a module that could be integrated in a labonachipbased pointofcare testing device which includes sample preanalytical cleanup and analysis
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