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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1007/s11258-014-0407-y

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1618-2650

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Quantitative analysis of specific target DNA oligo

Authors: Seung Pil Pack TaeHwe Heo Kamakshaiah Charyulu Devarayapalli Keisuke Makino
Publish Date: 2011/06/04
Volume: 401, Issue: 2, Pages: 667-676
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Abstract

Although a DNAimmobilized packedcolumn DNApacked column which relies on sequencedependent interactions of target DNA or mRNA in the mobile phase with DNA probes on the silica particle in a continuous flow process could be considered as an alternative platform for quantitative analysis of specific DNA to DNA chip methodology the performance in practice has not been satisfactory In this study we set up a more efficient quantitative analysis system based on a DNApacked column by employing a temperaturegradient strategy and DMSOcontaining mobile phase Using a temperaturegradient strategy based on T m values of probe/target DNA hybridizations and DMSO 5containing mobile phase we succeeded in the quantitative analysis of a specific complementary target distinguishable from noncomplementary DNA oligomers or other similar DNA samples In addition two different target DNA oligomers even with similar T m values were separated and detected quantitatively by using a packed column carrying two different DNA probes

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