Authors: Suquan Song Aibo Wu Xizhi Shi Rongxiu Li Zhixin Lin Dabing Zhang
Publish Date: 2008/03/27
Volume: 390, Issue: 8, Pages: 2141-2150
Abstract
Six molecularly imprinted polymers MIPs of erythromycin ERY were prepared by noncovalent bulk polymerization using methacrylic acid MAA as the functional monomer On the basis of binding analysis the MIPs with 12 optimum ratio of template to MAA were selected for subsequent scanning electron microscopy and Brunauer–Emmett–Teller analyses which indicated that the MIPs had more convergent porous structures than the nonimprinted polymers The equilibrium binding experiments showed that the binding sites of MIPs were heterogeneous with two dissociation constants of 0005 and 063 mg mL−1 respectively Furthermore the performance of the MIPs as solidphase extraction SPE sorbents was evaluated and the selectivity analysis showed that the MIPs could recognize ERY with moderate crossreactivity for other macrolides The overall investigation of molecularly imprinted SPE for cleanup and enrichment of the ERY in pig muscle and tap water confirmed the feasibility of utilizing the MIPs obtained as specific SPE sorbents for ERY extraction in real samplesThe authors acknowledge the financial support from the National “863’’ HighTech Project 2006AA10Z438 the National Key Project of Scientific and Technical Supporting Programs Funded by Ministry of Science Technology of China NO2006BAK02A09 Shanghai Nanotech Special Fund 0652nm017 and Shanghai Leading Academic Discipline Project B205 The authors are grateful to the Instrumental Analysis Center of Shanghai Jiao Tong University China for SEM photography We are grateful to Tom Hsiang University of Guelph Canada for technical language editing
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