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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer Berlin Heidelberg

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DOI

10.1007/bf01917295

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1618-2650

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Preparation of FeSubscript3/SubscriptOSubscri

Authors: Chengchao Chu Meng Li Long Li Shenguang Ge Lei Ge Jinghua Yu Mei Yan Xianrang Song
Publish Date: 2013/10/12
Volume: 405, Issue: 29, Pages: 9555-9561
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Abstract

We describe here the preparation of carboncoated Fe3O4 magnetic nanoparticles that were further fabricated into multifunctional core/shell nanoparticles Fe3O4CCNCs through a layerbylayer selfassembly process of carbon nanocrystals CNCs The nanoparticles were applied in a photoluminescence PL immunosensor to detect the carcinoembryonic antigen CEA and CEA primary antibody was immobilized onto the surface of the nanoparticles In addition CEA secondary antibody and glucose oxidase were covalently bonded to silica nanoparticles After stepwise immunoreactions the immunoreagent was injected into the PL cell using a flowinjection PL system When glucose was injected hydrogen peroxide was obtained because of glucose oxidase catalysis and quenched the PL of the Fe3O4CCNC nanoparticles The here proposed PL immunosensor allowed us to determine CEA concentrations in the 0005–50 ng · mL1 concentration range with a detection limit of 18 pg · mL1This work was financially supported by the Natural Science Research Foundation of China 51273084 51003039 21207048 21277058 the Natural Science Foundation of Shandong Province China ZR2012BZ002 and the Technology Development Plan of Shandong Province China grants 2012GGB01181 and 2011GGB01153

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