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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1002/chin.198940342

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1618-2650

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Tuning of Tidoped mesoporous silica for highly ef

Authors: Fuqiang Wang Zhonghua Shi Fan Hu Zhengrong Xia Ling Wang
Publish Date: 2012/11/20
Volume: 405, Issue: 5, Pages: 1683-1693
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Abstract

Extraction of phosphopeptides from rather complex biological samples has been a tough issue for deep and comprehensive investigation into phosphoproteomes In this paper we present a series of Tidoped mesoporous silica TiMPS materials with tunable composition and controllable morphology for highly efficient enrichment of phosphopeptides By altering the molar ratio of silicon to titanium Si/Ti in the precursor the external morphology Ti content internal longrang order and surface area of TiMPS were all modulated accordingly with certain regularity Tryptic digests of standard phosphoprotein α and βcasein were employed to assess the phosphopeptide enrichment capability of TiMPS series At the Si/Ti molar ratio of 81 the optimum enrichment performance with admirable sensitivity and capacity was achieved The detection limit for βcasein could reach 10 fmol and 15 phosphopeptides from the digest of αcasein were resolved in the spectrum after enrichment both superior to the behavior of commercial TiO2 materials More significantly for the digest of human placenta mitochondria 396 phosphopeptides and 298 phosphoproteins were definitely detected and identified after enrichment with optimized TiMPS material demonstrating its remarkable applicability for untouched phosphoproteomes In addition this research also opened up a universal pathway to construct a compositiontunable functional material in pursuit of the maximum performance in applications

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