Authors: W R Premasiri Ying Chen P M Williamson D C Bandarage C Pyles L D Ziegler
Publish Date: 2017/02/24
Volume: 409, Issue: 11, Pages: 3043-3054
Abstract
SERS spectra of 12 bacterial strains of urinary tract infection UTI clinical isolates grown and enriched from urine are reported A partial least squaresdiscriminant analysis PLSDA classification treatment of these SERS spectra results in strain level identification with 95 sensitivity and 99 specificity The classification model successfully identified the SERS spectra of a urinecultured strain not used to build this statistical model Enrichment was accomplished by a filtration and centrifugation protocol The predetermined drug susceptibility profiles of these clinical isolates thus allowed the SERS methodology to provide appropriate UTI antibiotic information in less than 1 h Most of this time was used for sample preparation procedures enrichment and washing for this proof of principle study SERS spectra of the enriched bacterial samples are dominated by nucleotide degradation metabolites adenine hypoxanthine xanthine guanine uric acid AMP and guanosine Strainspecific specificity is due to the different relative amounts of these purines contributing to the corresponding SERS spectra of these clinical isolates All measurements were made at the minimal bacterial concentration in urine for UTI diagnosis 105 cfu/mLThe relative contribution of each of the seven purines found to contribute to the bacterial SERS spectra are summarized in this bar graph Although strain specific differences are evident it can be see how the pattern of contributing purines is more different between the four species than between strains of a given speciesWe thank BD Life Sciences for providing the clinical isolates used in this study and for running the antimicrobial susceptibility testing on these clinical isolates BD and BD Phoenix are trademarks of Becton Dickinson and Company © 2016 BD The support of the NSF I/UCRC Collaborative Research Award Grant 1068070 is gratefully acknowledged
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