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Title of Journal: Anal Bioanal Chem

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Abbravation: Analytical and Bioanalytical Chemistry

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Springer-Verlag

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10.1007/s00024-015-1127-5

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1618-2650

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A clinical trial for therapeutic drug monitoring u

Authors: Tomoya Tachi Tetsunari Hase Yukihiro Okamoto Noritada Kaji Takeshi Arima Hiroyuki Matsumoto Masashi Kondo Manabu Tokeshi Yoshinori Hasegawa Yoshinobu Baba
Publish Date: 2011/08/13
Volume: 401, Issue: 7, Pages: 2301-
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Abstract

Microchip analysis is a promising method for therapeutic drug monitoring This led us to evaluate a microchipbased fluorescence polarization immunoassay FPIA system for pointofcare testing on patients being treated with theophylline The sera were collected from 20 patients being treated with theophylline Fluorescence polarization was measured on the microchip and theophylline concentrations in serum were obtained Regression analysis of the correlations was done between the results given by the microchipbased FPIA and the conventional cloned enzyme donor immunoassay CEDIA and between the results given by the microchipbased FPIA and the conventional particleenhanced turbidimetric inhibition immunoassay PETINIA We successfully carried out a quantitative analysis of theophylline in serum at values near its therapeutic range in 65 s The results obtained by the microchipbased FPIA correlated well with CEDIA and PETINIA results the correlation coefficients R 2 were 0986 and 0989 respectively The FPIA system is a simple and rapid method for pointofcare testing of drugs in serum and its accuracy is the same as the conventional CEDIA and PETINIA It is essential to use real samples from patients and to confirm good correlations with conventional methods for a study on the realization of microchipThis work was partially supported by Japan Society for the Promotion Science JSPS through its “Funding Program for WorldLeading Innovative RD on Science and Technology FIRST Program” and the GrantinAid for Scientific Research B 17310087 We thank Mr Kenji Uchiyama and Mr Yoshikuni Kikutani of the Institute of Microchemical Technology Co Ltd for valuable discussions on chip fabrication

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