Authors: P O Andrade R A Bitar K Yassoyama H Martinho A M E Santo P M Bruno A A Martin
Publish Date: 2006/10/10
Volume: 387, Issue: 5, Pages: 1643-1648
Abstract
FTRaman spectroscopy was employed to study normal human colorectal tissues in vitro with the aim of evaluating the spectral differences of the complex colon mucous in order to establish a characteristic Raman spectrum The samples were collected from 39 patients providing 144 spectra for the statistical analysis The results enable one to estabilish three welldefined spectroscopic groups of nonaltered coloretal tissues that were consistently checked by statistical clustering and biological histopathology analyses group 1 is represented by samples with the presence of epithelial layer connective tissue papillae and smooth muscle tissue group 2 comprises tissues with epithelial layer and connective tissue papillae group 3 presented mostly fatty and slack conjunctive tissue The study reveals the existence of an intrinsic spectral variability for each patient that must be considered when sampling tissues fragments to build a spectral database This is the first step for future studies and applications of Raman spectroscopy to optical biopsy and diagnosis of colorectal cancerThe authors thank the Colonoscopy Service of the Portuguese Charity Hospital of São José dos Rio Preto São Paulo the Coloproctology Department of the Medicine Faculty of São José do Rio Preto FUNFARME where surgeries were performed and the Vivalle Hospital of São José dos Campos SP for the samples supplement This work was supported by CNPq project 401018/20059 and FAPESP project 01/143848
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