Authors: Nicole Zehethofer Tom Scior Buko Lindner
Publish Date: 2010/10/03
Volume: 398, Issue: 7-8, Pages: 2843-2851
Abstract
This work reports on the fragmentation of phosphoinositides by tandem mass spectrometry MS/MS and MS³ experiments on a hybrid apexQe Fourier transform–ion cyclotron resonance mass spectrometer FTICR MS using internal infrared multiphoton dissociation IRMPD The fragmentation behavior of diacylphophatidylinositol triphosphate was intensively studied since an abundant loss of inositol biphosphate was observed This loss was suggested to occur via phosphate migration along the inositol head group Substantiation by MS³ experiments showed that this neutral loss is formed after the loss of water from the precursor ion indicating phosphate migration along the inositol ring to the glycerol backbone Further fragmentation of the ion formed by the loss of inositol biphosphate from diacylphophatidylinositol triphosphate resulted in the formation of a product ion with a molecular formula of C3H5O7P2 corresponding to a glycerol backbone linked to two phosphate groups We suggested different structures for this ion and compared their stability using modeling experiments
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