Authors: Jing Bao Wei Lv Yuyu Sun Ying Deng
Publish Date: 2013/02/23
Volume: 48, Issue: 12, Pages: 4223-4232
Abstract
In this study polycaprolactone PCL microfibrous scaffolds with berberine were fabricated to mimic the natural extracellular matrix ECM architecture and provide antimicrobial activity for annulus fibrosus tissue engineering Morphological characterization showed that there was a significant decrease of the average fiber diameter in the berberineloaded microfibrous scaffolds BMFS 040 ± 002 μm compared with that of the nondrugloaded microfibrous scaffolds MFS 189 ± 015 μm The antimicrobial activity drug release profile and biocompatibility of the scaffolds were evaluated The BMFS displayed excellent antimicrobial activities against Grampositive bacteria S aureus 6538 Gramnegative bacteria E coli 15597 fungus C albicans 10231 and drugresistant bacteria methicillinresistant S aureus BAA811 or MRSA BAA811 After seeding with porcine AF cells the in vitro biocompatibility of the scaffolds was determined by measuring cell attachment cell proliferation and ECM production Total cell number sGAG and collagen content gradually increased from day 1 to day 7 in both groups When compared to MFS the BMFS group displayed higher levels of cell proliferation throughout the experimental period These results indicate that PCL microfibrous loaded with berberine are novel biocompatible scaffolds with a broadspectrum antimicrobial activity for AF tissue engineeringThis research was partially supported by the Biomedical Engineering BME Program at the University of South Dakota The authors would also acknowledge the South Dakota Board of Regents Competitive Research Grant Award No SDBOR/USD 20111007 for financial support
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